linebreak

WET

Multiple frequency/solvent suppression
Smallcombe, Patt, and Keifer, JMR A (1995) 117:295
Patt, JMR (1992) 96:94 (in the Chem-Bio library)

  1. Preliminaries
    1. Sign the logbook (name and phone number)
    2. Login to the computer
        user name
        password
    3. Open Topspin (double-click the icon)
        topspin icon
  2. Acquire a 1H spectrum
    1. Is the shimming okay?
    2. Does the sample appear as expected?
    3. Calibrate the 90° pulse
    4. Optimize the acquisition parameters (sw, o1p, and td)

  3. Create a shaped pulse
    1. Type: stdisp
      the shape tool display window and interactive menu

      your
      spectrum
      appears in
      the upper
      right frame


    2. create a new excitation region:excitation region icon
      Adjust the region to one of the peaks of interest using the mouse
      to distribute the power evenly, the region widths should be equal
    3. From the "Manipulate" menu, select "calc shape from excitation"
      Manipulate pulldown menu
      The carrier frequency, the number of regions, and the left and right regions are determined by the expno opened and regions defined in the upper right panel of the shape tool

      calc shape from excitation window

      Select the following details:
        Shape (Seduce1)
        Flip Angle (90)
        Alignment w.r.t. (Center)

      click OK

      Note the pulse length and units (x104) - this will be "p11"
      Note down the equivalent length of a 90° square pulse
      Save the pulse Save-As icon and name it twice - OK

  4. Wet
    1. type: new
      use current parameters
    2. pulprog: wet
    3. p11 (set according to step 3, note the units)
    4. p16 2 ms (2000 us)
    5. power levels:
        type: edprosol
        choose standard soft-shape, 90°, square100.1000, enter the equivalent length from step 3 and calculate the power (note the value)
        exit without saving

        P = calculated power - 6 dB
        n = number of regions used in step 3

        sp7 = P + 0.87 + (6*n)
        sp8 = P - 1.04 + (6*n)
        sp9 = P + 2.27 + (6*n)
        sp10 = P - 5.05 + (6*n)
    6. shots:
        ns 8 (or a factor thereof)
        ds 16
    7. gradient pulses:
        gp21 80
        gp22 40
        gp23 20
        gp24 10
    8. run paropt on sp10, try 1 dB increments

  5. Acquisition
    1. type: rga
    2. or type: zg

  6. Process, Plot, Save
    DATA MAY BE DELETED at any time WITHOUT WARNING
    1. Open the my computer file icon on the desktop (double-click nmr500)
    2. Click the “start” icon to get to the nmrlab4 network shortcut
    3. Drag and drop files to nmrlab4.
      Preserve the hierarchy:
      directory/data/user/nmr/name/ expno

  7. Remove your sample & Log out
  8. Report problems and bugs to Yael or Shifi - 3748