1D Selective NOE

Nuclear Overhauser Effect

    new expno
  1. new
    1. start a new experiment
    2. update the expno
    3. choose: 1H_selnoe

  2. standard 1H NMR
    Change the pulse program to zg
    • (type: pulprog zg)
    Acquire a spectrum as usual:
    • sign the log-book
    • lock
    • shim
    • rga
    • zg
    • proc
    Tailor the standard parameters:
    • td, sw, sfo1 (and optional/optimal: p1, d1)

  3. determine the offset for on-resonance irradiation
    type: sr o1
    (that is the letter o and the number 1)
    put the cursor on the peak to irradiate and WRITE DOWN the value in Hz
    recalibrate the spectrum (or for an approximate calibration, type: sr 0)

  4. Increase the experiment number
    type: iexpno

    OPTIONAL NOTE: if you want to check the bandwidth/selectivity of the selective pulse (or you just want to check the offset magnitude), then run an extra experiment using the pulse program called "selzg" - use the offset determined in step 3 combined with the shaped pulse duration and power used in step 5 . . .

  5. Change the pulse program to the 1D selective NOE
    DPX200 or AV300, type: pulprog selno
    AV500 (use gradients!), type: pulprog selnogp

  6. Set the shaped pulse offset
    Open the acquisition parameters (type: ased)
    Scroll down the table DOUBLE CHECKing all your parameters
    d8 is the NOE mixing time (often between 150 - 800 ms, but longer or shorter values might also be appropriate)
    Note: *IF* you change sw, dw, or o1p at this point, then you will need to repeat steps 2 & 3 with the new values in order to redetermine the irradiation frequency (spoffs)
    set SPOFFS (shaped pulse offset) to the Hz value you wrote down in step 3

  7. Acquire
    check the experimental time (type: expt)
    click on the "play" icon or type: zg

  8. Process and print
    Multiple display mode .md with the standard NMR spectrum from step 2 can be helpful for analysis.
Report problems and bugs to Yael or Shifi - 3748